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 | Work in mouse ES cells could be replicated with human ES cells
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This discovery contradicts previously held views and could have wide-ranging implications for stem cell research. It is hoped the findings, from the Cambridge team lead by Professor Austin Smith and published in Nature, will lead to a better biological understanding of ES cells and more straightforward translation of detailed work done only in mouse ES cells to date.
Jason Wray, one of the authors on the paper, said: “That ES cells can self-replicate without external instruction implies that they are intrinsically programmed to self-renew. The new culture conditions will help us to understand the nature of the pluripotent state and how it might be manipulated to produce specialised cells in the laboratory.”
Since pluripotent mouse ES cells were first described they have been derived and grown using various combinations of feeder cells, conditioned media, cytokines, growth factors, hormones, foetal calf serum, and serum extracts. The thinking was that these provided the signals and instructions to stem cells to maintain them in an undifferentiated state and that without these the cells would specialise to become whatever type of cell they were prompted to become.
The MRC team were able to show that ES cells produce their own signalling molecules and that these signals are the key driving force behind differentiation - the process by which a stem cell gives rise to more specialised cell types. This means that while they still need to be grown in a culture giving them the sugars and proteins they need to stay alive, if these signalling molecules are eliminated or blocked, the ES cells can remain in their pluripotent state indefinitely.
A better understanding of what controls differentiation in mouse ES cells should also allow the work that has so far taken place only with mouse ES to be replicated in human ES cells.
Wray added: “ES cells with similar characteristics to mouse ES cells have yet to be derived from any other species. Last year it was shown that established human ES cells are more similar to mouse “Epi-stem cells”, which correspond to a later stage of development. We have reason to believe that what we have observed in mice will read across to other mammals and that the new culture conditions we have established may allow us to transfer our experience and techniques from animal models to human.”
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